In this study were analyzed biochemical parameters in a serum of brown trout (Salmo trutta) during and post spawning. For this purpose 25 fi sh in the spawning (Group I), 21 fi sh post-spawning (Group II) and 33 control fi sh (Group III) were used.Research has shown that during spawning signifi cant reduction in growth occurs, as well as mass loss, particularly in females. Signifi cant positive correlation between the total length and mass has been found. For all biochemical parameters between the three groups signifi cant differences were found (p<0.05). We have not found signifi cant differences in the values of serum glucose, cholesterol and chloride between Group I and II; however, protein, triglycerides, AST, sodium, potassium and calcium values were statistically signifi cant (p<0.05). For glucose, AST and calcium values no signifi cant differences between Group I and Group II were established. During spawning glucose, AST and calcium values were lower, while other biochemical parameters were increased.

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