Scopolamine impairs memory by causing oxidative stress in brain cells, especially the cortex and hippocampus. Medicinal plants with various effective compounds can have antioxidant effects. The aim of this study is to investigate the effect of the methanolic extract of Zirfon plant on oxidative stress and working memory impairment induced by scopolamine in male Wistar rats. In this experimental study, thirty-six male Wistar rats were randomly divided into six groups of 6. The control group, Scopolamine group (1 mg/kg), Extract Group (300 mg/kg), and Extract groups 100, 225, and 300 mg/kg together with scopolamine which received extracts as gavage and scopolamine intraperitoneally for 10 days. After performing the behavioral test of the New Object Identification Test (NORT), hippocampal and cortex tissues were sampled to measure the activity of catalase (CAT), superoxide dismutase (SOD), and acetylcholinesterase (AChE). Our results showed that injection of scopolamine reduces the discrimination index in NORT. The dose of 300 extracts had the highest effect, so even in its administration together with scopolamine, a significant (p<0.001) reduction in the number of working memory errors was created. Biochemical findings in hippocampus and Cortex Tissues showed that scopolamine decreased the activity of antioxidant enzymes (CAT and SOD) and increased the activity of AChE. Treatment with Zirofen extract restored the activity of SOD, CAT, and AChE. The results of this research indicate that Zirfon extract has a strengthening effect on working memory and generally exerts its effect through antioxidant activity.

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