The goal of this investigation was to assess thepotential probiotic properties of 10 lactic acid bac.teria (LAB)isolated from vaginal microflora of 60healthy women in Kirsehir province. The selectedLAB (lactic acid bacteria)were identified by 16SrDNA gene sequencing. In order to evaluate theiprobiotic properties, low pH and high bile salt.concentration tolerance,antagonistic and antimicrobial activities, HO, production capabilities aswell as auto-aggregation and coaggregation capa.bilities,cholesterol removal, their capacity to adhere to uroepithelial cells and to determine theiranti-proliferative or cytotoxic effects on HeLaHuman Cervical Carcinoma Cell and Caco-2Human Colon Carcinoma Cell Linel cells wereinvestigated. All strains were found to be resistanto low pH (pH 2.5 and 3.0)conditions and high bilsalt concentrations (0.3%,0.5%, and 1.0%). Vagi.nal LAB displayed a wide spectrum of antimicrobi.al activity against urogenital pathogens, and resistance to antibiotics commonly prescribed foinfections caused by these pathogens. All isolatestested were able to auto aggregate (ranging from47% to 81% assessed at 4 hours of incubation) andto co-aggregate with the three pathogenic microorganisms. The highest antiproliferative action on theHeLa and Caco-2 cells was found to vary between75-95% at the highest doses of the metabolite prod.ucts of the Ll, L6 and L8 strains. Considering althese properties together with, it is thought thatthese strains can contribute to the development ofnew probiotic formulations which may be effectivein the prevention and treatment of urogenital infec.tions.

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