Fermentation tank refers to the device used in industry for microbial fermentation. Its main body is generally a main cylinder made of stainless steel plate, and its volume ranges from 1m3 to several hundred m3. In the design and processing, attention should be paid to the tight and reasonable structure. Can withstand steam sterilization, have certain operating flexibility, minimize internal accessories (avoid dead spots), strong material and energy transfer performance, and can be adjusted to facilitate cleaning and reduce pollution, suitable for the production of multiple products and reducing energy Consume.
1. Use in the pharmaceutical industry: According to fermentation engineering skills, a variety of drugs have been developed, such as human growth hormone, recombinant hepatitis B vaccine, monoclonal antibodies of certain varieties, interleukin-2, anti-hemophilia factors, etc.
2. Use in the food industry: There are three main types of goods, one is the production of traditional fermented goods, such as beer, fruit wine, vinegar, etc. .; the second is the production of food additives; the third is to help solve food problems.
3. Use in the field of environmental science: strengthening of microorganisms in sewage treatment.
The main body of the fermentation tank is provided with an interlayer, a thermal insulation layer, which can be used for heating, cooling and thermal insulation. Both the tank body and the upper and lower elliptical heads are processed by spinning R angles. The inner wall of the tank is mirror-polished and cleaned without dead ends. The fully enclosed design ensures that the materials are always mixed and fermented in a pollution-free state. The equipment is equipped with air breathing holes, CIP cleaning nozzles, manholes, inlets and outlets.
The fermentation tank equipment adopts the internal circulation method. The mixing paddle is used to disperse and break the air bubbles. It has a high rate of dissolved oxygen and good mixing effect. The tank is made of SUS304 or 316L imported stainless steel, and the tank is equipped with an automatic spray cleaning head to ensure that the production process meets GMP requirements. Fermenters are widely used in dairy, beverage, bioengineering, pharmaceutical, fine chemical and other occupations. In recent years, with the continuous progress of science and technology, the fermentation profession as a link between the national economy and the people’s livelihood has developed rapidly, and the fermentation equipment has also been updated from traditional to modern.
Below we introduce the use steps of the fermentation tank:
Install the electrodes. Before sterilizing the fermenter, check each interface, whether the appearance of each pipeline is normal, and whether the steam outlet pipe is firm. Each valve should be checked by two persons for complete closure. At least two people are required to be present during sterilization, one person is responsible for operating various valves, and the other person is responsible for observing and reporting various parameters. After confirming that each valve is closed, open the steam generator water inlet valve, start the steam generator, and the steam generator starts to automatically enter water. At this time, you should open the drain valve at the lower part of the steam generator, and close the drain valve after removing water rust as much as possible. . Observe the water level in the steam generator to prevent accidents.
At this time, the jacket air outlet valve of the fermentation tank should be fully opened, the main valve of the steam generator should be slowly opened, and part of the jacket air inlet valve should be slowly opened, and water vapor should be introduced into the jacket. At the beginning, the steam pressure may be unstable, and the pressure gauge pointer swings violently. You can properly close the small jacket inlet valve, and then open the sampling valve steam valve, the sampling valve inlet steam valve, the bottom valve outlet steam valve, and the bottom valve inlet steam. Valves, sterilize the sampling valve and the bottom valve, while exhausting unstable water vapor. When the water vapor pressure does not change violently, close the bottom valve and the air outlet valve in turn.
The temperature inside the fermentation tank gradually rises. When the temperature is set to 90 °, the stirring motor stops rotating. At this time, open the main inlet valve of the tank, open the tank outlet valve, and open the following valves in sequence: Slowly open the steam inlet valve of the tank to introduce steam into the tank. The gauge pressure of the air filter starts to rise. When the pressure exceeds 0.1MP, open the condensate valve to drain water. Slowly rise, adjust the air outlet regulating valve to reach the temperature and pressure required for sterilization, and the timer will start when it reaches the sterilization temperature.
Adjust the inlet valve, steam inlet valve and gas outlet adjustment valve of the fermenter jacket to keep the temperature and tank pressure at the set temperature. After the sterilization time has expired, close the steam inlet valves that have been opened, close the main steam valve, completely release the exhaust valve in the tank to release the pressure in the tank, and turn off the power to the steam generator. Turn on the air compressor, and when the tank pressure drops to 0.05MP, slowly open the intake regulator to maintain a positive pressure in the tank.
During the sterilization process of the fermentation tank, the operator should wear long trousers and protective gloves to prevent burns. If the steam generator automatically feeds water during the sterilization process, the total steam pressure will decrease rapidly. If necessary, you can open a large steam valve to maintain the pressure, but you must carefully observe the pressure change. When the tank is vented with steam, the tank pressure drops rapidly after sterilization. Pay attention to the timing of passing sterile air. Try not to make the air compressor and steam generator work at the same time. The air filter pressure should not exceed 0.2MP during sterilization. The size of the condensate will affect the concentration of the liquid in the tank after sterilization, and the increase of steam condensate should be considered.