Stainless Steel Fermenter, Mechanical Stirring Bioreactor


Stainless steel Fermenter Systems Description

Stainless steel Fermenter system mainly includes tank body, pipe valve, air filtration system, temperature automatic control system, speed control system, pH online detection and automatic control system, DO online monitoring and automatic control system, automatic defoaming, automatic feeding, PLC centralized Control system, host computer control system, etc. Oil-free silent air compressor, automatic electric steam generator.

Design pressure 0.3 Mpa; body material: 316L stainless steel, jacket 304 stainless steel; with special sampling valve for fermentation tank, bottom discharge valve, with side large viewing angle mirror, 12V safety Philips lighting, with temperature, pH, DO , Defoaming port, acid and alkali replenishment port, inoculation port, replenishment port, air inlet, exhaust port, seed transfer port, liquid level electrode, defoaming electrode, etc.; internal polishing accuracy Ra0.4, external polishing accuracy Ra0. 6. All welds are firm, neat and beautiful.

Stainless steel Fermenter Technical Specifications

No. Item name

Specifications

  Model

BIOF-S10L

BIOF-S20L

BIOF-S30L

BIOF-S50L

BIOF-S100L

1. Tank capacity

10L

20L

30L

50L

100L

Tank size

Φ180×400mm

Φ230×500mm

Φ273×550mm

Φ300×650mm

Φ400×750mm

Jacket size

Φ220×3mm

Φ273×3mm

Φ325×3mm

Φ350×3mm

Φ450×4mm

Tank
  • Sterilization in place, design pressure 0.3 Mpa;
  • Body material: 316L stainless steel, jacket 304 stainless steel;
  • With special sampling valve for fermentation tank, bottom discharge valve, with side Large-view mirror, 12V safety Philips lighting, with Temperature, pH, DO, defoaming port, acid and alkali replenishment port, inoculation port, feed port, air inlet, exhaust port, seed transfer port, liquid level electrode, defoaming electrode, etc.;
  • Internal polishing accuracy Ra0.4, external polishing accuracy is Ra0.6, and all welds are firm, neat and beautiful.
2. Motor power

0.4kw

0.75kw

1.0kw

1.0kw

1.5kw

Speed range

50~600rpm ±1%

50~600rpm ±1%

50~600rpm ±1%

50~600rpm ±1%

50~600rpm ±1%

Mixing system
  • Servo motor, speed governor; continuous and stable, adjustable; control accuracy ±1%
  • Top-type mechanical stirring system, adopts special 316L stainless steel for stirring shaft, AD mechanical seal (a special mechanical seal system that can meet higher fermentation requirements);
  • Adopts adjustable 2-stage 6-inclined blade stirring paddle, 1-stage compression type high-efficiency Bubble paddle, 4-level detachable baffle;
3. Intake & filtration
  • Deep ventilation is adopted, with the maximum ventilation 1.5VVM;
  • Primary filtration, 0.01μm precision sterilization filter, fine filter selection of high-quality products, to ensure safety and independent sterilization, greatly extending the service life; annular gas distribution at the bottom Device
4. pH control
  • Electrode: Mettler high temperature resistant glass electrode
  • Control range: 2-12pH, display range: 0-14pH,
  • Control accuracy: ±0.02pH, resolution: 0.01pH;
  • Control: Intelligent PID control, abnormal pH value alarm, segment control, data can be uploaded to the host computer
5. DO2 control
  • Electrode: Mettler high temperature resistant glass electrode
  • Control range: 0-100%, display range: 0-200%,
  • Control accuracy: ±5%, resolution: 0.1%;
  • Control: Intelligent PID control, abnormal DO value alarm, data can be uploaded to the host computer
6. Temperature Control System
  • Electric heating, water cooling, circulating pump temperature control, air escape function and water cut, over-temperature protection function, with good heat exchange efficiency;
  • Intelligent PID control not only ensures the rapidity of temperature control, but also realizes energy saving.
  • Fermentation temperature control range: cooling water temperature +6~50℃; accuracy: ±0.2℃; resolution: 0.1℃;
7.       Defoaming system
  • Antifoaming agent: flow addition by peristaltic pump
  • Highly sensitive defoaming electrode
  • Control method: time and proportion automatic control
8.       Flow detection system
  • Stainless steel flowmeter controls the intake air flow
  • Control method: manual control
9.       Pressure detection system
  • Direct-mounted pressure gauge
  • Range: 0-0.4MPa
  • Control method: manual control
10.  Automatic feeding system
  • Equipped with a complete replenishment system, the replenishment can be measured;
  • Number of peristaltic pumps can be selected according to needs, and the conventional configuration is four.
  • The role of the peristaltic pump can be selected and set on the control interface, which makes the user more convenient and flexible in actual use.
  • Equipped with specially designed replenishment pins to ensure the safe operation of replenishment.
11.  Sterilization system
  • Manual control, the sterilization method includes jacket heating, and steam sterilization in the tank at the same time; or the tank does not enter the steam and sterilizes only by jacket heating
  • Sterilization temperature: 100-130℃;
12.  Transplant
  • Can be transferred to the next level, using stainless steel valves and polished pipes;
  • The rationality of the design ensures the safe realization of the transfer
13.  System equipped with valve pipeline
  • Valve material: AISI 316L, the valve in contact with the material adopts imported diaphragm valve
  • The pipeline is made of stainless steel with precision polished; argon gas shielded welding; meets GMP requirements
  • The stainless steel table frame is used, and the bottom is installed with pulleys for easy movement
14.  La-M controller & LF-Control control system
  • Siemens PLC programmable controller, advanced IoT touch screen, multi-touch operation
  • Online display, control and editing of all parameters, historical data recording and export, support user customization, and can also be customized according to user requirements
  • It can simultaneously detect and control the stirring speed, tank temperature, pH value, DO value, feeding, acid and alkali, defoaming, etc.;
  • Each parameter has PID adjustment process display, such as set value, real-time value, PID set value, upper and lower limit value;
  • The upper and lower limits of temperature, rotation speed, pH value, DO value can be set, and the limit alarm function is provided;
  • Speed, temperature, pH value, DO value, replenishment, etc. can be set to automatic, manual and closed states;
  • There is a complete feeding system, the peristaltic pump can periodically and quantitatively feed, the feeding can be measured, and the curve can be displayed;
  • With the function of over-level alarm and automatic refilling, with the function of over-foam level alarm and automatic addition of defoamer;
  • Equipped with the associated control of dissolved oxygen and speed, that is, the method of changing the speed to improve the dissolved oxygen concentration of the culture solution;
  • Associated control of feeding and pH; Associated control of feeding and dissolved oxygen.
  • Sterilization manual control, the sterilization method includes jacket heating, and steam sterilization in the tank at the same time; or the tank does not enter the steam and sterilizes only by jacket heating, sterilization temperature: 100-130℃;
  • The system has real-time display, data recording, data storage, data analysis (histogram, curve
  • Line graphs and batch reports) output printing, password management, abnormal analysis and other functions and alarm functions;
  • The historical data and curves of various parameters can be stored for many years (you must have a host computer to achieve); important
  • The parameters are written into the database synchronously to prepare for data analysis at any time. The real-time data can be restored intact when the power is cut off and the machine is restarted; the full Chinese interface is provided with detailed help instructions.
  • The parameters of each detection and control loop can be manually set and corrected online on the screen, temperature, PH, and DO sensors can be adjusted and adjusted, and the flow rate of each peristaltic pump can be calibrated;
  • The system has self-adaptive and self-diagnostic capabilities; abnormal conditions can be automatically alarmed and protected according to customer settings.
  • All alarm accidents can be recorded and consulted;
  • Operation has a password protection function, the password is divided into operator and administrator level passwords;
  • With automatic control and manual control switching.


Optional parts

Can lid lift Equipped with a high-power precision cylinder to automatically lift the tank cover (50-500L) by 300-500mm and fix it, which is convenient for cleaning and maintenance
SIP automatic sterilization function One-button automatic sterilization, including filters, tanks, and pipelines. Manual sterilization of sampling valve and discharge valve
Tank weighing system The weighing module is used to accurately measure the fermentation liquid with an accuracy of up to 0.1%
Automatic detection and control of air flow Vogtlin mass flow controller: The mass flow controller is used to accurately control the air intake flow, and the air flow can be automatically adjusted according to the dissolved oxygen value. The maximum design flow is about 2VVM, and the control accuracy is ±0.5%
Pressure automatic control system Adopt high-temperature resistant high-precision pressure sensor to accurately detect the pressure of the fermentation tank, and the regulating valve automatically adjusts the tank pressure. Measuring range: 0-0.35Mpa, ±0.3%, control accuracy: ±0.5%


Stainless steel Fermenter Main parts brand

SN Part Name Brand Origin Remark
1. pH electrode Mettler Switzerland Standard fitted
2. DO electrode Mettler Switzerland Standard fitted
3. pH signal transmission line Mettler Switzerland Standard fitted
4. DO signal transmission line Mettler Switzerland Standard fitted
5. touch screen MCGS Well-known domestic brands Standard fitted
6. PLC Siemens Germany Standard fitted
7. Thermostatic water pump WILO German brand Standard fitted
8. Electric machine Panasonic Japan Standard fitted
9. Frequency converter Panasonic Japan Standard fitted
10. controlling software BIOF-10L type China Standard fitted
11. The electromagnetic valve Sanlixin/Julang Zhejiang/Shanghai Standard fitted
12. Pneumatic diaphragm valve, pneumatic angle seat valve, manual diaphragm valve, proportional solenoid valve Baode/Gemi Germany\China Standard fitted
13. Rotameter Chengfeng China Standard fitted
14. The filter cartridge Shanghai Standard fitted

Product video :https://youtu.be/A8aLhtbOc3c

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